Abstract : In the present research work non effervescent floating matrix formulation of Misoprostol by using various polymers. Initially analytical method development was done for the drug molecule. Absorption maxima was determined based on that calibration curve was developed by using different concentrations. Floating agent accurel concentration was optimized. Then the formulation was developed by using different concentrations of polymers of various natural and synthetic polymers. The formulation blend was subjected to various preformulation studies, flow properties and all the formulations were found to be good indicating that the powder blend has good flow properties. Among all the formulations the formulations prepared by using Methocel K15 were unable to produce desired drug release, they were unable to retard drug release up to 12 hours. The formulations prepared with karaya gum  retarded the drug release up to 12 hours in the concentration of  7.5 mg (F6).The formulations prepared with Sodium CMC were also retarded the drug release for more than 12 hours. Hence they were not considered. The optimized formulation dissolution data was subjected to release kinetics, from the release kinetics data it was evident that the formulation followed zero order mechanism of drug release.