Abstract- The literature currently describes many various methods of creating Platelet-Rich- Plasma (PRP); however, according to Robert Marx’s widely accepted definition a platelet rich solution is three times (3x) greater in platelet concentration compared to the same volume of whole blood with a double centrifugation process is PRP. In this experiment the creation of PRP was optimized using two previously published PRP protocols from Messora et. al. (2011). Furthermore, the literature minimally describes the possibility of activating cytokine and vesicular release from PRP using collagen scaffolds for full thickness wound repair. To date, there are publications that discuss the collagen scaffold and PRP combination therapy use in connective tissues repair, but this leaves out epithelial tissue repair where PRP could also assist in the healing response. Here the creation of an electrospun collagen scaffold is created and used to activate the optimized PRP on-demand. PRP levels were validated through manual cell counting methods and flow cytometry. Once quantitative assessment of the PRP was completed, activation of the PRP took place. This activation process was validated throughlight-microscopy (LM) and scanning-electron-microscopy(SEM).
Dr. Hafiza Amna Aman , Dr. Muhammad Qasim Ali , Dr. Muhammad Umar Salim
Platelet-Rich- Plasma, light-microscopy, scanning-electron-microscopy