Objective: To investigate the anti atherosclerotic activity of ethanol extract of Anisomoles Malabarica in male Wistar rats. Material & method: – In this model of atherosclerosis,  30  adult  male  wistar  rats  were  evenly divided into 5 groups. Group-1 and Group-2 served as untreated and model controls respectively, while Group-3, 4 and 5 were the treatments groups which were simultaneously treated with standard, 200 and 400 mg/kg  extract respectively along with  High Fat Diet.  On  last day, blood samples for biochemical parameters, were obtained under inhaled diether anaesthesia. Results: – HFD caused atherosclerosis as evidenced by marked elevation in Cholesterol, Triglycerides, LDL, VLDL and decrease in HDL levels. Co-administration of extract with HFD decreased rise Cholesterol, Triglycerides, LDL, VLDL and increase in HDL levels. .Conclusion:  –  It  was  observed  that  the  ethanol   extract   of  Polygonum   galbrum conferred Anti- atherosclerotic activity by biochemical observation against HFD induced atherosclerosis in rats. In the near future could constitute a lead to discovery of a novel drug for treatment of drug induced atherosclerosis.