Anisomeles malabarica, a plant species commonly found in the Western Ghats of India, has been traditionally used in various folk remedies to treat various ailments. However, the scientific validation of its medicinal properties is limited. The present study aims to investigate the anti-cholinesterase and antioxidant potential of leaf extract of Anisomeles malabarica.The leaf extract was prepared using ethanol as a solvent and evaluated for its anti-cholinesterase activity using the Ellman’s assay. The antioxidant potential was assessed using the 1, 1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging assay and the ferric reducing ability of plasma (FRAP) assay. The results showed that the leaf extract exhibited significant anti-cholinesterase activity, with an IC50 value of 12.6 μg/mL, which was comparable to that of the standard drug, galantamine. The antioxidant activity of the leaf extract was also found to be significant, with a DPPH radical scavenging activity of 83.2% and a FRAP value of 1.43 mmol/L. The study suggests that the leaf extract of Anisomeles malabarica may have potential therapeutic applications in the treatment of cholinergic disorders, such as Alzheimer’s disease, and may also be useful in scavenging free radicals and protecting against oxidative stress.