ABSTRACT: Calophyllum inophyllum is used for the treatment of various diseases, namely inflammation, arthritis, diabetes, bacterial infection, etc. We planned to isolate the active constituents of ethanol extract of Calophyllum inophyllum leaves and evaluated their anti-inflammatory and anti-arthritis activity. The petroleum ether, ethanol and aqueous extract were prepared from leaves of Calophyllum inophyllum, and screened for photochemical analysis. The different flavonoid compound isolated from ethanol extract, and further evaluated their anti-inflammatory and anti-arthritis activity. The maximum phytoconstituents along with flavonoids were observed in ethanol extracts of leaves of Calophyllum inophyllum. The eight different fractions (F1 to F8) were isolated from ethanol extracts of Calophyllum inophyllum leaves. The F6 and F7 indicate the presence of flavonoids, tannins & phenolic compounds. The findings indicate that the F6 and F7 isolated from Calophyllum inophyllum leaves extract exhibited moderate to highly anti-inflammatory and anti-arthritis activity. It suggests that the anti-inflammatory and anti-arthritis activity of F6 and F7 due to the presence of polyphenol and flavonoids.